Coding

Part:BBa_K1982010:Design

Designed by: Zexu Li   Group: iGEM16_NEU-China   (2016-10-09)


CRY2-VP64(Eukaryotic LACE system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 411
    Illegal BglII site found at 870
    Illegal BamHI site found at 1349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 295
    Illegal AgeI site found at 1024
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 647
    Illegal BsaI.rc site found at 56
    Illegal SapI.rc site found at 164


Design Notes

no


Source

CRY2 and CIB1 are extracted from Arabidopsis thaliana. tCas9 is from GE Share company. VP64 is synthetic construct.

References